Abstract
Studies were made to characterize the cholinesterases from erythrocytes, plasma and homogenates of cardiac ventricular muscle and brain of guinea pigs as specific or non-specific cholinesterases (ChEs). The relative content of both can be estimated using the substrates acetylcholine, acetyl-β-methylcholine and butyrylcholine. The content of non-specific ChE was found to decrease in the sequence: plasma cardiac-ventricular-muscle brain. The inactivation, reactivation and ageing-rate of the various ChEs were determined and yielded the following differences: 1. The specific ChEs from erythrocytes and brain were inhibited to different degrees by obidoxime. 2. The non-specific ChEs from plasma and cardiac ventricular muscle could be clearly differentiated from that of brain by means of their different k2-values upon inactivation with DFP. 3. The specific ChE of brain once inactivated by DFP was found to age more slowly than that of erythrocytes, taking the reactivation by obidoxime as indicator. 4. The reactivation of the phosphorylated non-specific ChEs and the half-life of the ageing processes were found to be dependent on the obidoxime concentration used.
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