Abstract
Characterization of bonded-amine packing was achieved with an organic-aqueous eluent by use of aromatic compounds as standard samples. The results indicate that the main interaction between the stationary phase and the solute is hydrogen bonding, hydrophobicity of the packing being negligible. The amine packing was also employed for an analysis of carbohydrates. A colorimetric detection of a reduced form of Tetrazolium Blue served for the determination (limit of detection: 10 ng of saccharide). The separation time was reduced by gradient elution. An oligosaccharide of d.p. 20 was eluted in less than 40 min.
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