Characterization of Antifungal Metabolites Produced by Aeromonas Hydrophila and Analysis of its Chemical Compounds Using GC-MS

Abstract

Bioactives were analyzed using gas chromatography-mass spectroscopy (GC-MS) techniques, then the in vitro antibacterial and antifungal activity of the methanolic extract was evaluated. Twenty three bioactive compounds were identified in the methanolic extract of Aeromonas hydrophila. GC-MS analysis of Aeromonas hydrophila revealed the existence of the Tricyclo[4.3.1.1(3, 8)]undecan-1-amine 1-Methyl-4-[nitromethyl]-4-piperidinol, Bicyclo[2.2.1]heptan-2-one, 4, 7, 7-trimethyl-, semicarbazone, D-Streptamine, O-6-amino-6-deoxy-α-Dglucopyranosyl-(1–4), 4-(2, 5-Dihydro-3-methoxyphenyl)butylamine, 3-Cyclohex-3-enyl-propionic acid, Benzenemethanol, 2-(2-aminopropoxy)-3-methyl-, Benzene, 1-methyl-4(4-morpholyl)ethenylsulfonyl-, Indan1, 2-dione, 4-methyl-, 2, 3-Dihydroindole-4-ol-2-one, 5, 7-dibromo-3, 3-dimethyl-, Benzamide, 3-amino-N-[4, 5dihydro-5-oxo-1-(2, 4, 6-trichlorop, 2-Acetonyl-9-[3-deoxy-β-d-ribouranosyl]hypoxanthine, DL-Leucine, Nglycyl-, 3-(2, 5-Dimethylanilinomethyl)-5-(3-fluorobenzylidene)-2, 4-thia, Benzenepropanoic acid, α(hydroxyimino)-, Butanoic acid, 4, 4′-dithiobis [2-amino-, [S-(R*, R*)]-N-Propionyl-D-glucosamine Actinomycin C2, N-(O-Nitrophenylthio)-l-leucine, D-Fructose, diethyl mercaptal, pentaacetate, 1H-Indole, 4-(3-methyl-2butenyl)-, Spiculesporic acid, 2, 5-Piperazinedione, 3, 6-bis(2-methylpropyl). Nerium olender (Alkaloids) was very highly active (5.408±0.24) mm. The results of anti-fungal activity produced by Aeromonas hydrophila showed that the volatile compounds were highly effective to suppress the growth of Aspergillus flavus (6.009±0.22). Aeromonas hydrophila produce many important secondary metabolites with high biological activities. Based on the significance of employing bioactive compounds in pharmacy to produce drugs for the treatment of many diseases, the purification of compounds produced by Aeromonas hydrophila can be useful.