Characterization of Antifungal Secondary Metabolites Produced by Klebsiella pneumoniae and Screening of its Chemical Compounds Using GC-MS

Abstract

Bioactives were analyzed using gas chromatography-mass spectroscopy (GC-MS) techniques, then the in vitro antibacterial and antifungal activity of the methanolic extract was evaluated. Twenty two bioactive compounds were identified in the methanolic extract of Klebsiella pneumoniae. GC-MS analysis of Klebsiella pneumoniae revealed the existence of the 6,9,12-Octadecatrienoic acid , phenylmethyl ester , (Z,Z,Z)-, 5,7-Dodecadiyn-1,12-diol, 1,4 Decadiyne, 10,12-Octadecadiynoic acid, 1-Cyclopropyl-3,4-epoxyhex-5-en-1-yne, N,N-Dimethyl-3-methoxy-4-methylphenethylamine, Ethenetricarbonitrile , 3,4-xylidino, Pentyl glycolate, 3-(1,1'-Biphenyl-4-yl)butanenitrile, 4'-Amino-6-methoxyyaurone, Ethanone , 2,2'-(octahydro-2,3-quinoxalinediylidene)bis[1-phe, 1,1'-Bicyclohexyl , 4-methoxy-4'-propyl-, [1.4]Bipiperidinyl-4'-carboxamide , 1'-(chlorobenzenesulfony, 7H-Pyrrolo[2,3-d]pyrimidin-4-amine ,Vinylsulfonamide, 1-Phenyl-2-(4-methylphenyl)diazene 1-oxide, N-Benzyl-N-ethyl-p-isopropylbenzamide, 1-phenyl-2-(4-methylphenyl)-diazene 1-oxide, 1-Benzylindole, Isophthalic acid , di(2-methoxyethyl) ester, 1-Tert , butyl -3,3-bis(trifluoromethyl)diaziridine, 4-Dehydroxy-N-(4,5-methylenedioxy-2-nitrobenzylidene)tyram. Datura stramonium (Alkaloids) was very highly active (6.481±0.24) mm. The results of anti-fungal activity produced by Klebsiella pneumoniae showed that the volatile compounds were highly effective to suppress the growth of Aspergillus flavus (6.287±0.30). Klebsiella pneumoniae produce many important secondary metabolites with high biological activities. Based on the significance of employing bioactive compounds in pharmacy to produce drugs for the treatment of many diseases, the purification of compounds produced by Klebsiella pneumoniae can be useful.