Abstract
Digital holographic microscopy (DHM) combined with microfluidics provides high-throughput label-free imaging flow cytometry for generating physical data from heterogeneous samples like particle or cell mixtures. For reliable analysis of the sample fluid, e.g., for cell counting, it is crucial that all particles in the sample fluid flow are monitored within the field of view of the optical microscope camera. In this work we combine a DHM system with a microfluidic system that allows lateral hydrodynamic focusing within a rectangular microfluidic channel. We investigate the hydrodynamic focusing effect at different settings of the microfluidic pump system by analyzing the retrieved DHM quantitative phase images of living cells flowing through the hydrodynamically-focused sample fluid flow. Our results show that DHM represents a feasible tool for the label-free characterization of sample fluid flows in microfluidic systems with hydrodynamic-focusing capabilities.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
