Characteristics of insulin-like growth factor- I and receptor expression in Diabetic Foot

Abstract

Objective This study investigated the distribution and expression of the IGF- Ⅰ and its receptor 2 subunits ( αt, β) in non-diabetic and diabetic patients' normal skin as well as diabetic foot-ulcer, to clarify the expression of level change and diabetic foot ulcer inherent connection. Methods the serum and foot skin of diabetes foot patients (30 cases), diabetes patients (23 cases)and control (25 cases)ware collected. After fixation、dehydration、 embedding、 slicing, the line IGF-1、 IGF-Ⅰ Rαand IGF-Ⅰ Rβimmunohistochemistry. observed the results of immunohischemistry under light microscope at 400 times. Results IGF- Ⅰ、IGF- Ⅰ Rαand IGF- Ⅰ Rβis positive in non-diabetic or diabetic patients' normal skin and diabetic foot ulcer, but the variation is different. IGF- Ⅰ positive cell rate of DM group than in normal control group decreased ( P ＞ 0. 05 ) , Diabetic foot ulcer group than in the expression of further weakening in simple diabetic group (P ＞ 0. 05 ). IGF- Ⅰ Rαand IGF- Ⅰ Rβpositive cell rate of DM group than in normal control group decreased (P ＞ 0. 05 ), Diabetic foot ulcer group than in the expression of further weakening in simple diabetic group ( P ＞ 0. 05 ). Conclusion IGF- Ⅰ diabetic wound the lack or insufficiency may be caused by diabetes, endothelial cell damage, and prolonged healing of diabetic foot ulcers are directly related. Wound expression of IGF- Ⅰ Rαand IGF- Ⅰ Rβ decreased, and biological activity of the reduced impact of cytokine induced signaling and gene expression in the nucleus, resulting in lost control and wound healing of chronic dermal ulcer formation. Key words: Insulin like growth factor Ⅰ; Insulin like growth factor Ⅰ ; Diabetic foot ulcers