Abstract
This chapter reviews the isolation and identification of brassinosteroids (BS). Isolation of BS in a pure form is tedious and time consuming. Sensitive and highly specific biological tests play an important role in guiding the correct choice of active fractions in the course of the isolation process. The identification of BS in plant raw material is a complex and multistep process of extraction, chromatographic purification, monitoring of the biological activity of the fractions, and other operations. Because of the very low BS content in plants, it is not always possible to isolate them in quantities that are sufficient for the recording of all spectral characteristics. In such cases, the identification of BS has to be done by making derivatives and comparing them with separately synthesized authentic samples. Gas chromatography, in combination with mass spectrometry (GC-MS), is employed in most cases for the identification of natural BS.
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