Abstract
This chapter deals with the biosynthesis and metabolism of brassinosteroids (BS). BS biosynthesis takes place in normal cells, but less intensively. Cultured cells of Catharanthus roseus proved to be especially useful for biosynthetic studies of BS, because brassinolide and castasterone are produced in amounts that are comparable with those observed for pollen or immature seeds. Campesterol is the starting material for the biosynthesis of brassinolide in view of the similarity of their carbon skeletons. Tremendous progress has been achieved in the understanding of many subtle details of the biosynthesis of BS and their possible mode of action in the course of investigations with Arabidopsis thaliana L. mutants. The metabolism of castasterone and brassinolide was studied on explants of mung bean vigna radiata seedlings. It was demonstrated that castasterone was converted into unknown metabolites in this plant rather than into brassinolide. Incubation of mung bean with brassinolide 1 led to the formation of a new glycoside.
Published Version
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