Chapter 7 - HPLC resolution using polysaccharide derivatives as CSP

Abstract

This chapter discusses the high-performance liquid chromatography (HPLC) resolution using chiral stationary phases (CSPs) for estimating both, enantiomer composition and obtaining pure enantiomers. Chiral compounds can be resolved by using CSPs. Therefore, the key factor of this technique is how we choose a suitable CSP for the resolution of a target compound. The gas chromatography (GC) using a CSP is effective for volatile compounds of relatively low molecular weight and is mostly used for analytical purposes. On the other hand, the HPLC method using a CSP is suitable for compounds with a relatively high molecular weight. The resolution by HPLC will maintain its major role in the future. Chiral HPLC is a practically useful separation technique not only for determining optical purity, but also for obtaining optical isomers. Polysaccharide-based CSPs are the most popular CSPs today, and can achieve the successful resolution of a wide range of chiral compounds particularly by optimizing the separation conditions. The chiral recognition mechanism of the polysaccharide-based CSPs has been investigated on the basis of chromatographic methods, and spectroscopic and computational approaches at a molecular level. The classification of the chiral recognition mechanism on the CSPs will enable the selection of suitable resolution conditions and further development of more effective CSPs.