Abstract
This chapter discusses the use of filter paper supports for plant cultures. The use of ashless filter paper supports has been recommended for the culture of isolated plant tissues of the callus type. These tissues are cultured according to Gautheret's technique, which consists in culturing explants, 2–3 mm side measurement, weighing about 150 mg, in vertical tubes containing about 25 ml of nutrient medium generally solidified with agar, as these tissues cannot tolerate immersion in a liquid. These paper supports can be made very quickly and placed in position and 100 tubes can be equipped with them in one-quarter of an hour. It is observed that if the support is lowered too far into the liquid, a bubble of air remains enclosed under the support. Growth in a liquid medium surmounted by a filter paper support is less abundant than on agar. Culture on ashless filter paper is suitable not only for tissue cultures, but also for the culture of embryos, seedlings and any other plant material not too large in size, and which it is required to culture aseptically on a nutrient medium of strictly calculated composition.
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