Abstract
After pretreatment, enzymatic hydrolysis aims for the maximum conversion of the polymeric and oligomeric carbohydrates of lignocellulosic biomass into fermentable sugars for the formation of value-added products including biofuel. This chapter focuses on several important aspects of enzymatic hydrolysis of biomass, including a detailed understanding of the reaction mechanism involved with cellulase, hemicellulase, and ligninolytic enzymes. Moreover, the role of the cellulose binding domain and catalytic domain in facilitating the enzymatic hydrolysis of biomass is discussed. Further, different substrate and enzyme-related factors are critically analyzed for a detailed understanding of the complex reaction system with the biomass. Readers of this chapter will acquire an indepth insight on several critical phenomenona for enzymatic hydrolysis with the significant progress that has attempted to transform laboratory-scale information into large-scale reality.
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