Abstract

This chapter discusses the components and accessories for preparative high-performance liquid chromatography. Chromatography is primarily a separation technique. If it is applied for the collection of pure material then it is called preparative chromatography, and if one fractionates with the aid of a pump on fine-particle columns, it is called modern preparative liquid chromatography. The aim is to extract as much material in as pure a state as possible. In order to satisfy this aim, two chromatographic variants have to be taken into consideration: (a) batch or conventional liquid chromatography; (b) continuous liquid chromatography. In batch chromatography, one phase moves and the other remain stationary. In continuous chromatography, the conventional stationary phase is moved countercurrent in a "moving bed" against the mobile phase.

Full Text
Published version (Free)

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call