Abstract
H/u curves for columns of increasing internal diameter are shown to be useful for evaluating their liquid chromatography (LC) performance. In particular, the H/u curve reveals whether the packing technique or the packing material is defective. H/u curves give peak dispersion against mobile phase velocity. In preparative high performance liquid chromatography (prep-LC) an important point is the evaluation of column performance and of column packing quality. The chapter considers four parameters of prep-LC, which are usually changed compared to analytical LC: the increased column internal diameter, the increased particle size, the increased particle size spreading, and changes in the derivatization chemistry of the packing materials. It discusses the influence of these parameters on the plate number and the H/u curves, and evaluate the performance and quality of prep-LC systems. The influence of increased particle size on the H/u curve is important for practical prep-LC.
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