Abstract
Publisher Summary This chapter describes the properties of the Rous Sarcoma Virus (RSV) vectors and their use in expressing recombinant proteins in chick embryo fibroblasts (CEFs) and other cultured avian cells. This expression system has significant experimental advantages and has been invaluable for the structure–function analysis of the human transferrin receptor (TR). Various related RSV vectors have now been generated and can be used to express recombinant proteins in cultured cells and in chickens. RSV is the only known replication-competent retrovirus that carries a host-derived oncogene, src . Because the virus does not need the host-derived src oncogene for replication, src can be removed and replaced by other genes. This feature of RSV enables it to be used as a replication-competent helper-independent retroviral vector. The major advantage of the RCAS (RC, replication-competent; A, avian leukemia virus long terminal repeat; S, with splice acceptor) vectors is the fact that they are replication-competent retroviral vectors containing all the viral genes necessary to produce infectious virus. The RCAS vectors are suitable for unregulated high level expression of recombinant proteins in cultured CEFs.
Published Version
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