Abstract
The optimum production of proteins is currently a crucial necessity. Mammalian cells have been used more frequently in recent years for recombinant protein production as well as for antibodies, viruses, viral-subunit proteins, and vectors for gene therapy. Recombinant protein expression systems that use mammalian cells can introduce appropriate protein folding, posttranslational modifications, and product assembly, all of which are necessary for its full biological activity. These covalent changes may alter the protein’s clinical effectiveness or produce characteristics that are useful for biochemical evaluation. Proteins generated in mammalian cell lines are quality-controlled by a procedure that selectively inhibits the entry of misfolded, incompletely folded, and unassembled proteins into the secretory pathway. In this chapter, mammalian expression system, various mammalian cell lines that express the gene, and various vector systems that deliver the gene to mammalian cells have all received significant attention. Additionally covered are the media, cell culture format, and current methods for creating recombinant cell lines. Also addressed are ways to increase productivity and prospects for developing mammalian cell lines in the future. The key takeaway from this chapter is that methods, expression systems, and applications of gene expression in mammalian cell lines should be made available.
Published Version
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