Abstract
The ability to produce recombinant proteins by utilizing different “cell factories” revolutionized the biotherapeutic and pharmaceutical industry. Chinese hamster ovary (CHO) cells are the dominant industrial producer, especially for antibodies. Human embryonic kidney cells (HEK), while not being as widely used as CHO cells, are used where CHO cells are unable to meet the needs for expression, such as growth factors. Therefore, improving recombinant protein expression from mammalian cells is a priority, and continuing effort is being devoted to this topic. Non-coding RNAs are RNA segments that are not translated into a protein and often have a regulatory role. Since their discovery, major progress has been made towards understanding their functions. Non-coding RNA has been investigated extensively in relation to disease, especially cancer, and recently they have also been used as a method for engineering cells to improve their protein expression capability. In this review, we provide information about methods used to identify non-coding RNAs with the potential of improving recombinant protein expression in mammalian cell lines.
Highlights
The ability to produce recombinant proteins by utilizing different “cell factories” revolutionized the biotherapeutic and pharmaceutical industry, and influenced health care operations worldwide [1]
We provide information about methods used to identify non-coding RNAs with the potential of improving recombinant protein expression in mammalian cell lines
We provide a discussion of the methods that are used to identify non-coding RNAs with the potential of improving recombinant protein expression in mammalian cell lines
Summary
The ability to produce recombinant proteins by utilizing different “cell factories” revolutionized the biotherapeutic and pharmaceutical industry, and influenced health care operations worldwide [1]. Improving recombinant protein expression from CHO cells is a priority and continuing effort is being devoted to this since the first therapeutic protein, human tissue plasminogen activator, was approved [6]. Approaches such as improving metabolism, glycosylation, anti-apoptosis and pro-proliferation, molecular chaperones, and protein folding have been successfully implemented [7,8]. Small non-coding RNAs are primarily short RNA segments that are not translated into a protein. We provide a discussion of the methods that are used to identify non-coding RNAs with the potential of improving recombinant protein expression in mammalian cell lines
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