Abstract
Thin-layer chromatography is a method of separating and isolating nonvolatile compounds. Like other chromatography techniques, this one uses a mobile phase and a stationary phase. In this context, it is a thin covering of absorbent material such as aluminum oxide, silica gel, or cellulose. This layer is applied on inert substrate sheets made of plastic, glass, or aluminum foil. The mobile phase in the TLC method is a solvent or a solvent mixture. The experiment is performed on a sheet of aluminum foil, plastic, or glass that has been coated with a thin layer of adsorbent material. The most frequent materials used are aluminum oxide, cellulose, and silica gel.
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