Abstract
In molecular biology, the quantification of nucleic acids is frequently used to estimate the content of DNA and RNA in a combination. A sample of nucleic acids with the necessary quantity of optical performance is used in future reactions or procedures. Due to the presence of conjugated double bonds between the constant purine and pyrimidine bases, DNA and RNA both exhibit strong UV absorbance. These molecules have an OD (optical density) of absorbance maximum at 260nm that is linearly related to the concentration of DNA in the solution up to the OD value of 2. DNA purity is examined using the spectroscopic approach. The nucleic acid extracts' greatest absorbance is at 280nm, where proteins are the main impurities.
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