Abstract
This chapter discusses carbohydrate analysis by liquid chromatography (LC) and supercritical fluid chromatography (SFC) using evaporative light-scattering detection. LC has developed rapidly over the past 20 years, and significant advances have been made in all areas of instrumentation. Improvement of detection sensitivity was a great necessity, particularly in universal detection. Evaporative light-scattering (ELS) detection is a recent detection technique increasingly used in high-performance liquid chromatography (HPLC) and SFC of various compounds, including carbohydrates. ELS detection acts as a qualitative or quantitative visualization of each separated solute only if the solute is in a liquid or solid state after the vaporization process. The presence of interfering products in complex media necessitates investigations into sample preparation and the selection of the chromatographic system compatible with a suitable detection. Improvements in the pollution and/or security aspects can be made using a micronebulizer because the flow rate of a solvent can be seriously reduced.
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