Abstract
The analytical characteristics of a commercial evaporative light-scattering detection (ELSD) system were compared to those of condensation nucleation light-scattering detection (CNLSD) for the measurement of polyethylene glycol and polyethyl oxide samples with molecular masses ranging from 1000 to 45 000. Results for CNLSD with both an in-house constructed detector and a commercial condensation particle counter (CPC) were included in the study. Using a flow injection mode, limits of detection (LODs) for CNLSD were improved by up to a factor of over 1000 compared to ELSD. LODs with CNLSD for size-exclusion chromatography with an aqueous mobile phase were on average 130 times better with both the in-house detector and CPC, with average LODs of 15 ng/ml. While response for ELSD is typically nonlinear, the linear range for response of CNLSD was at least three orders of magnitude when the CPC was used for CNLSD, and for the in-house constructed detector when diffusion screens were employed. The polyethylene glycols tested in this study provided similar response on a mass basis with CNLSD, but 40% and 70% lower response was observed for a dextran and a polyacrylic acid, respectively.
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