Abstract
This chapter describes the theory and practice of high-speed microbore high performance liquid chromatography (HPLC). The chapter also explores the absolute limits to separation speed as predicted by well-established theory. The development of high-speed HPLC systems has received less attention than gas chromatography. They were able to achieve complete resolution of seven aromatic compounds on microbore packed columns (1 m I.D.) within 20 to 30 seconds. The continuing interest in microbore HPLC (6–10) and its application to high-speed separations gives the impression that microbore HPLC is intrinsically faster than larger bore columns. It can become more obvious from the equations presented that bore size has little effect upon separation speed. Some performance advantages of narrow-bore columns are becoming evident, such as reductions in the wall effects or improved dissipation of frictional heat. In addition, because columns can be efficiently coupled, shorter column segments can be connected to produce column lengths closely matched to separation needs. Microbore HPLC is attractive for high-speed HPLC for a variety of practical, rather than theoretical reasons.
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