Abstract
The effect of viscous heat dissipation on efficiency in high-speed high-performance liquid chromatography was investigated for columns packed with non-porous 1.5 μm particles. When these columns are operated at high flow-rates and therefore high-pressure drops, viscous heat is generated leading to axial and longitudinal temperature gradients within the column which are translated into secondary gradients of the mobile phase viscosity, the flow velocity, the diffusion rate, and the retention factor. This resulted in additional band broadening which can destroy the separation quality as demonstrated by chromatograms and plate height curves. Thermostatting of the eluent to a specific temperature below the column wall temperature compensated for this effect. The necessary degree of precooling decreased with increasing column temperature.
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