Abstract
Chapter 3 contains the information related to the use of the most suitable separation techniques used in metabolomics research, considering also sample preparation as an important sample pre-treatment step fundamental to perform the analytical separation. General principles of the most used extraction methods and analytical separation techniques are described. In this context, the main focus will be on column chromatography, briefly discussing theoretical principles and practical issues, along with instrumental features. A section on multidimensional chromatography is also present.
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