Chapter 3 - Biofilm formation and antibiotic susceptibility profile among bacterial strains detected in urinary tract infected patient

Abstract

The multidrug-resistant bacteria organize biofilm in a systematic way to resist the action of prescribed antibiotics. The biofilm is established on the surfaces of medical implants used for the treatment. In this context, patients introduced with catheter tubes, often infected with strains Gram-positive and Gram-negative bacteria, suffer long-term infection due to resistance conferred by bacterial candidates. The bacteria adopt a sedentary lifestyle, inside the biofilm and evade the action of antibiotics along with other drug-resistant mechanisms. This present pursuit aims at detecting multidrug-resistant bacteria, in the biosample of urinary tract infected patient undergoing treatment in a local hospital named Veer Surendra Sai Institute of Medical Sciences and Research, Burla, Odisha, India. The conventional biotyping and antibiogram techniques were used. The bacterial strains were identified as Escherichia coli (BME1), Klebsiella pneumoniae (BMKl1), and Staphylococcus aureus (BMS2). The bacterial strains were subjected to biofilm assays as well as antibiotic susceptibility tests. The degree of biofilm formation was strongly correlated with absolute resistance toward the test antibiotics as well as higher MIC values.