Abstract
This chapter discusses problems relating to the interpretation of spectroscopic data in the presence of degradation products. The spectrophotometric measurement of organic compounds that absorb in the ultraviolet region of the spectrum is contaminated by errors when degradation products or other chemical species that also absorb are present. Whatever the sensitivity of the spectrophotometer being used, the simple measurement of the extinction, even at maximum absorption, does not take into account merely the concentration of the product being determined. This is because of the additivity of absorptions for the different constituents of a mixture. The interfering absorption can be linear in the form of a continuous background, or its graphical representation as a function of wavelength measured, can be a curve with maxima and minima. The chapter highlights that in the laboratory; a third of the time devoted to spectrophotometry determinations is taken up in extracting results from experimental data; and much re-checking is necessary, because the possibilities for error are so numerous. It is not possible, to increase the number of measurements ad infinitum, in an attempt to increase the accuracy, because the workload in interpretation of the experimental data would become disproportionate to the result obtained.
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