Abstract
Currently, the primary challenge to modern agricultural science is to meet the global demand for products and ensure food security for the growing population. No doubt the conventional plant breeding techniques and use of agrochemicals to obtain greater crop productivity and variety have been established to fight against biotic and abiotic stress in plants for better yield of agricultural products. The scientific community is exploring better techniques that can satisfy the need of mankind without affecting the ecosystem. Genetic engineering in plants is a recent advancement in the field of plant biotechnology and is more precise and can quickly obtain the desired trait in plants. This technology majorly focuses on improving the crop yield and quality by expressing desirable genes that are responsible for desirable traits such as tolerance to extreme conditions, herbicide, pest resistance, and enhanced secondary metabolite production, which is of medical importance. Nanomaterial-mediated genetic transformation is one reliable and efficient means of crop improvement for sustainable development in agricultural science. This technology has revolutionized modern agriculture as they are used as an effective delivery system to plants. Chitosan-based nanoparticles find their best application as nano-carrier due to their intrinsic properties such as cationic, biocompatible, high loading capacity, and good penetration potential with good release kinetics. Thus chitosan nanoparticles are used to deliver different types of genetic materials such as DNA, RNA, miRNA, siRNA, pDNA, CRISPR/Cas9 single guide RNA for plant transformation. This chapter provides an overview of chitosan nanoparticles as a delivery system and with a focus on their application as a safe genetic delivery system in plants.
Published Version
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