Abstract
Callus tissues of root origin synthesize a greater amount of alkaloids in the dark, and those of leaf origin in light. For the tissue culture of tropane alkaloid plants, a modified culture medium after Murashige–Skoog solidified with agar is used. For quantitative determination of tropane alkaloids, titration in water-free medium and tropeoline amphi-indicator is used. For qualitative determination, the following methods are used—thin-layer chromatography, gas chromatography, and autoradiography. The total alkaloid content of the intact plant is found to be higher than that of the tissue cultures, which originates from leaf, stem, or root. Under the influence of illumination, the alkaloid content of the callus tissues of root and leaf origin increases twofold over levels in cultures grown in the dark.
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