Abstract

Microfluidic devices, especially microchips for electrophoresis, are very attractive tools for clinical and pharmaceutical analysis. Moreover, electrochemical detection has proven to be very effective for microfluidic electrophoresis chips because of characteristics such as inherent miniaturization, sensitivity, low cost, portability, and compatibility with microfabrication technology. Thus, these devices enable the injection, separation, and detection of the compounds of a complex sample in a very short time, enhancing some of the features of conventional capillary electrophoresis. In this experimental approach, an overview of the optimization of an analytical methodology based on commercial microfluidic electrophoresis chips and portable instrumentation is provided. The optimal configuration is achieved for the separation and detection of uric acid and related compounds. The feasibility of the methodology is demonstrated through the fast analysis of urine samples. This method avoids the use of traditional enzymatic reactions and other labile and expensive reagents.

Full Text
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