Chapter 13 - RDT and genetic engineering: Basic of RDT method, PCR, and application

Abstract

In the past century, recombinant DNA technology (RDT) was only an idea that certain traits in live beings may be altered by manipulating the expression of target genes. This field has made significant contributions to human progress in the modern age. This technology has a wide range of applications and has the potential to improve many elements of life, such as changing health, improving food resources, and building resistance to a variety of detrimental environmental effects. Critical proteins required for health concerns and dietary needs can be produced safely, inexpensively, and inadequate quantities using this technique. Bioentrepreneurs look for commercial value in the technologies they utilize to undertake biotechnology research. Entrepreneurship and biotechnology are closely connected. The scientists Stanley Cohen of Stanford University and University of California San Francisco created the first rDNA molecules and this marks the beginning of genetic engineering. The most essential tool utilized by geneticists is rDNA. There would be no genetic engineering without rDNA technology. In recent years, a considerable number of articles in the business literature have studied biotechnology at the regional, corporate, and individual levels. This book chapter will encourage an entrepreneur to explore the fascinating field of biotechnology. This chapter gives insight into the fundamentals of RDT, genetic engineering, polymerase chain reaction and its applications, as well as the relationship of these technologies with bioentrepreneurship and the recombinant DNA technology market-growth and trends are also discussed.