Abstract

High-performance liquid chromatography (HPLC) is a separation technique, which can be applied to analyze compounds of different samples. Depending on the nature, chemical structure, and molecular weight of the analytes, it is possible to select the type of HPLC. In this sense, different kinds of HPLC have emerged to allow qualitative and quantitative information regarding individual components of the sample under study. The knowledge of these kinds of components is the basis to detect manipulations and fraudulence for profiteering, evading the law set in the interest of food authenticity. The aim of this chapter is to describe HPLC principles and different methods as a tool for the qualitative and/or quantitative identification of the components present in a specific sample which could be use as fingerprints in the authentication process.

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