Abstract
Alignment of the y-axis is a helpful alignment tool for all spectra when used for both quantitative and qualitative measurements using spectroscopy. The assumptions of regression are that the data channels or x-axis of data is aligned and that only amplitude in the y-axis changes with concentration in some mathematically defined manner. An aligned y-axis provides an improved method to compare spectra and to relate amplitude to concentration. The correction of y-axis (amplitude) variation provides a minimum bias between instruments and provides a platform for improved calibration transfer between instruments. This chapter provides a discussion and presentation for using photometric reference materials to align the y-axis for ultraviolet, visible, NIR, IR, and Raman intensity spectroscopic methods. A general method for correcting each type of absorption-based spectrophotometer is given as well as the types of reference standards used for calibration or verification of the photometric scale. For Raman spectroscopy a method is described for intensity correction.
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