Abstract

Alignment of the x-axis (i.e., the wavelength or wavenumber axis) is assumed for all spectra used for the purposes of quantitative and qualitative work. Note that the assumption for regression modeling as well as spectral comparison algorithms is that the data channels, or x-axis scales of the spectroscopic data, are precisely aligned and that only amplitude in the y-axis changes with respect to concentration in a predefined mathematical relationship. This chapter provides a discussion and set of spectra to illustrate the use of reference materials to align the x-axis for the major types of optical spectroscopy, to include the UV, visible, NIR, IR, and Raman spectroscopic methods.

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