Abstract
Protein expression is the crucial step in the overall process of protein characterization. After the cloning and transformation/transfection into host cell, the researcher needs to optimize the various parameters for protein expression. Various physical, chemical, and biological parameters are considered influencing the protein expression directly or indirectly. Standard protocol for all the protein expression optimization is not available till date. Every protein in unique and a separate protocol needs to be developed for individual protein’s expression. Here in this chapter, the authors briefly describe the important parameters in detail that should be considered for protein expression optimization to enhance the protein production.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
