Abstract

Citric acid is produced through submerged culture fermentation of sugar cane molasses. The modified growth rate model known as the Leudeking–Piret equation, incorporating yield of biomass and product, can express citric acid production. In addition, a logistic model is able to describe the growth kinetics of Saccharomycopsis lipolytica. The logistic equation defines the lag phase and the exponential and stationary phases. The logistic growth kinetic model successfully predicted the batch culture for citric acid production. The maximum specific growth rate and maximum cell concentration as kinetic parameters of an initial substrate (glucose) concentration of 50gL−1 were 0.25h−1 and 4.71gL−1, respectively. The model can project a rate constant of 1.6g(Lh)−1 for an initial cell concentration of 100mgL−1 and an incubation period of 10h.

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