Chapter 1.1 - Simultaneous Determination of 653 Pesticide Residues in Teas by Solid Phase Extraction (SPE) with Gas Chromatography–Mass Spectrometry (GC–MS) and Liquid Chromatography–Tandem Mass Spectrometry (LC–MS/MS)

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An efficient and sensitive method has been established for simultaneous determination of 653 pesticides in teas by GC–MS and HPLC–MS/MS. The method involved extraction with acetonitrile followed by cleanup using Cleanert-TPT SPE and subsequent identification and quantitation of 490 pesticides by GC–MS and 448 pesticides by HPLC–MS/MS. The LODs for pesticides determined by GC–MS were between 1.0 and 500 μg/kg, and those determined by HPLC–MS/MS were between 0.03 and 4820 μg/kg. At the low fortification levels of 0.01–100 μg/kg, the average recoveries of 94% of the pesticides determined by GC–MS were between 60 and 120%, 77% of which had an RSD below 20%. For 91% of pesticides determined by HPLC–MS/MS, the average recoveries were between 60 and 120%, 76% of which had an RSD below 20%. The paper also reports a novel SPE column, Cleanert TPT, which comprised graphitized carbon black (PestiCarb), polyamine silica, and amide polystyrene for purifying the tea samples. The results indicated good repeatability and reproducibility.