Abstract
Liquid chromatography-mass spectrometry (LC–MS) is a powerful analytical technique used for separation, identification, and quantification of both unknown and known compounds as well as to elucidate the structure and chemical properties of different molecules. It is very useful for analyzing small molecules and offers higher sensitivity and selectivity in the trace analysis of multicomponent containing substances. This chapter deals with several aspects of LC–MS, starting from its basic components like ionization sources, mass analyzer, detectors to statistical methods for data analysis. In addition, some major application of LC–MS in medicinal plant research has been discussed in this chapter.
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