Abstract
In this chapter, a phytotoxin that is secreted by the bacterium Pseudomonas syringae is studied. Instructions are provided for quantification of the growth of the bacteria, removal of the bacteria from the culture, and extraction of the toxin from the culture. The toxicity of this substance is assayed on the leaves of a plant. The yield is quantified with spectrophotometry, using ultraviolet radiation. The structure is examined with infrared spectroscopy, liquid chromatography–mass spectrometry, and nuclear magnetic resonance spectroscopy. In each exercise, background on theory and techniques is provided. A list of the materials that are needed and a step-by-step procedure are also included. Most of the exercises conclude with a guide for evaluation of the results and some questions that should be considered.
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