Abstract
Precautions for the safe handling of microorganisms in a laboratory are presented. The two biosafety levels that are most pertinent to the exercises in this book are summarized. The personal protective equipment required to work safely with chemicals in a laboratory is described. The use of safety data sheets to understand the hazards of chemicals is explained. Summaries of the hazards presented by the chemicals that are used in the exercises in this book are given. The proper way to use laboratory devices to avoid accidents is discussed. The materials necessary for containing and cleaning spills of chemicals are mentioned. Instructions are given for the proper response to emergencies in the laboratory such as contact of skin or eyes with chemicals, injury, nausea, fainting, fire, and earthquake. A guide to creating a written record of the plan for each experiment and summarizing the results in a laboratory notebook is provided.
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