Abstract
The development of recombinant DNA technology, the methodology used to join DNA from different biological sources for the purpose of determining its sequence or for manipulating its expression, spawned an era of gene discovery and launched an industry. This chapter covers the mathematics involved when using restriction endonucleases and ligase. The chapter shows how to calculate the optimal concentration of DNA fragment ends for the most efficient ligation reactions. Calculations relating to packaging of recombinant DNA molecules into phage lambda vectors, transformation of bacterial cells by recombinant plasmids, and screening for recombinant clones by hybridization to complementary sequences are discussed. This chapter shows how to determine the size of DNA fragments by gel electrophoresis and how to calculate the size of deletions generated using the BAL 31 nuclease.
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