CHANGES OF THE INDICES OF LIPID PEROXIDATION AND ANTIOXIDANT SYSTEM IN LUNGS UNDER CONDITIONS OF EXPERIMENTAL BRONCHIAL ASTHMA WITH CHRONIC PERIODONTITIS AND THEIR CORRECTION BY TIOTRIASOLIN

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In this paper, it has been shown that the model process of bronchial asthma (BA) with chronic periodontitis (CP) is accompanied by an increase of lipid peroxidation system indices (LPO) compared with the control group: the content of malonic dialdehyde and conjugated dienes; initial increase with further decrease in the activity of the AOS enzymes: superoxide dismutase, catalase, the content of ceruloplasmin in the lungs of guinea pig males on the 4th, 18 th, 25 th day of the experiment. These changes indicate the development of oxidative stress in the formation of BA with CP and the corrective effect of thiotriazolin.Objective. Determination of some indices of lipid peroxidation (LPO) and antioxidant system (AOS) in the lungs of the guinea pigs in the modeling process of BA with CP on 4th, 18th, 25th day of the experiment and effect of thiotriazoline on them.Material and methods. Experiments were conducted on 50 guinea pigs (males), with body weight 0,25-0,27 kg. Animals were divided into five groups, ten animals in each. Intact guinea pigs constituted the first group. Animals with experimental BA at CP without thiotriazoline correction constituted the second, third, fourth group respectively on the 4th, 18th, 25th day of the experiment before the correction, fifth group included animals with BA at CP on the 25th day of simulation of the disease after correction with thiotriazoline. Experimental BA was reproduced by V. I. Babych method. Chronical periodontitis was reproduced by O. N. Voskresens'kyy method. The level of conjugated dienes was determined by the method of V. B. Havrylov and M. I. Myshkorudna; the level of malondialdehyde was determined by the method of E. N. Korobeynikov: superoxide dismutase level activity was determined by the method of R. Fried; the level activity of katalase was determined by the method of B. Holmes, C. Masters; ceruloplasmin level activity was determined by the method of V. G. Kolb and V. S. Kamyshnikov. Statistical methods of processing the results of the study were processed by the method of variation statistics using Student's criterion.Results and discussions. The results of the studies showed changes in the parameters of the prooxidant-antioxidant system depending on the periods of the formation of BA with CP without correction by thiotriazoline: an increase in the levels of LPO in all of the days of the experiment, the initial increase with a further decrease in the activity of the AOS enzymes; decreasing of levels of LPO indices and increase in activity of AOS enzymes after correction by thiotriazolinom.Conclusions. The obtained results indicate significant changes in the parameters of the prooxidant-antioxidant system in the lungs of experimental animals with BA and CP and the antioxidant effect of thiotriazolin.