CHANGES IN THE CONTENT OF IMMUNOGLOBULINS AND CIRCULATING IMMUNE COMPLEXES IN THE BLOOD SERUM OF RABBIT RECIPIENTS DURING ALLOGENEOUS WHOLE BLOOD TRANSFUSION

Abstract

The paper presents the results of research on changes in the content of immunoglobulins and circulating immune complexes in the blood serum of recipient rabbits after allogeneic whole blood transfusion. The relevance of the work is determined by the importance of monitoring the state of immunoglobulins and CIC, since these indicators inform about the success of the blood transfusion procedure and the removal of the material by the recipient's immune system. Thus, the aim of the work was to study changes in the content of immunoglobulins and CIC in the blood serum of recipient rabbits. The material for research was blood serum samples obtained from 5 rabbits on 3, 8 and 23 days after transfusion. In the experiments, clinically healthy animals were used, which were transfused with whole blood. The feeding diet of the experimental animals met the need for nutrients and biologically active substances, the animals had free access to water. Blood from donor animals was collected from the jugular vein using a semi-closed method. Donor blood samples of rabbits were prepared in polymer containers with the anticoagulant Tsfda. Keeping animals, transfusions, manipulations and conducting research were carried out on the basis and in the conditions of Vedmedservis NSC, Animal Blood Bank NNL and the Department of Surgery and Pathophysiology named after Acad. I.O. Povazhenka, NULES of Ukraine. The study of the content of immunoglobulins in the blood serum of rabbits was carried out according to the Mancini method, the CIC was determined by the method based on the selective precipitation of high molecular weight immune complexes contained in the blood serum with polyethylene glycol with a molecular weight of 6000Da, followed by the determination of the optical density by spectrophotometry at λ = 450 nm. Based on the results of the research, it was established that with allogeneic transfusion of whole blood to recipient rabbits, there is a significant increase in the content of immunoglobulins of class M in blood serum on the 3rd day of the experiment and a decrease in their content on the 23rd day of the experiment. It was shown that with allogeneic transfusion of whole blood to recipient rabbits, the content of immunoglobulins of class G and A in blood serum decreases during the 23 days of the experiment. Allogeneic transfusion of whole blood in the body of recipient rabbits activates the formation of circulating immune complexes in the blood serum of animals, which can be deposited in the perivascular space and cortical layer of the kidneys, causing complement activation and inflammatory processes.