Changes in T lymphocyte subsets accompany the clinical response to Mesenchymal Stem Cell (MSC) therapy � first experience in irradiation-induced colitis

Abstract

Event Abstract Back to Event Changes in T lymphocyte subsets accompany the clinical response to Mesenchymal Stem Cell (MSC) therapy – first experience in irradiation-induced colitis Jan Voswinkel1*, Jean-Jacques Lataillade2, Sabine Francois3, Mohammad Mohty1, Norbert C. Gorin1, Marc Benderitter3 and Alain Chapel3 1 Université Pierre et Marie Curie, Paris, Hôpital Saint Antoine, APHP, Haematology and Cell Therapy, France 2 Burn Treatment Center, Percy Military Hospital,, France 3 Radiological Protection and Human Health Division, Institute of Radiological Protection and Nuclear Safety, France Background: Therapy by Mesenchymal Stem Cells (MSC) facilitates functional recovery and dampens the systemic inflammatory response in radiation-induced colitis. Bone marrow-derived MSC from the patients´ children were injected to four patients. Patients and Methods: MSC were obtained by culture from bone marrow aspirates. A quantity of 2x106 - 6x106 MSC /kg were infused intravenously to the patients. Pain, hemorrhage, frequency of diarrheas and fistulisation as well as the lymphocyte subsets in peripheral blood were evaluated before MSC therapy and during the follow-up. Results: Two patients revealed a substantiated clinical response for pain and hemorrhage after MSC therapy. In one patient pain reappeared after 6 months and again substantially responded on a second MSC infusion. A beginning fistulisation process could be stopped in one patient resulting in a stable remission for more than 3 years of follow-up. The frequency of painful diarrhea diminished from an average of 6/d to 3/d after the first and 2/d after the 2nd MSC injection in one patient. A decline of CD4+ and CD8+ T lymphocytes and an increase of potentially regulatory CD25+ T cells accompanied the clinical response in this patient after the MSC injections. In all patients prostate cancer remained in stable complete remission. No toxicity occurred. Conclusion: A modulation of the lymphocyte subsets towards a regulatory pattern and diminution of activated T cells accompanies the clinical response in refractory irradiation-induced colitis. For patients with refractory chronic inflammatory and fistulising bowel diseases, systemic MSC injections represent a safe and effective option for salvage therapy. Keywords: lymphocyte immunology, Stem Cells, MSc, Colitis, Treg, cell therapy Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Translational immunology and immune intervention Citation: Voswinkel J, Lataillade J, Francois S, Mohty M, Gorin NC, Benderitter M and Chapel A (2013). Changes in T lymphocyte subsets accompany the clinical response to Mesenchymal Stem Cell (MSC) therapy – first experience in irradiation-induced colitis. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00039 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 08 Mar 2013; Published Online: 22 Aug 2013. * Correspondence: Dr. Jan Voswinkel, Université Pierre et Marie Curie, Paris, Hôpital Saint Antoine, APHP, Haematology and Cell Therapy, Paris, France, jvoswinkel@aol.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Jan Voswinkel Jean-Jacques Lataillade Sabine Francois Mohammad Mohty Norbert C Gorin Marc Benderitter Alain Chapel Google Jan Voswinkel Jean-Jacques Lataillade Sabine Francois Mohammad Mohty Norbert C Gorin Marc Benderitter Alain Chapel Google Scholar Jan Voswinkel Jean-Jacques Lataillade Sabine Francois Mohammad Mohty Norbert C Gorin Marc Benderitter Alain Chapel PubMed Jan Voswinkel Jean-Jacques Lataillade Sabine Francois Mohammad Mohty Norbert C Gorin Marc Benderitter Alain Chapel Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.