Abstract
We measured the degree of nucleosome formation at the gene promoters in trichostatin A-treated (1, 2, and 3 µg/mL) cells of the archiascomycete Saitoella complicata and those in enlarged S. complicata cells after zymolyase treatment. TSA-treated and enlarged cells showed similar changes in nucleosome occupancy in five out of six positions in the gene promoters. These results suggest that changes in nucleosome formation at the gene promoters could serve as stress response mechanisms elicited in response to spheroplast (zymolyase treatment) and TSA treatment. In addition, we demonstrated that changes in nucleosome position occurred mainly in cells treated with 1 µg/mL TSA, whereas cells treated with 2 and 3 µg/mL TSA did not exhibit significant changes in the degree of nucleosome formation.
Highlights
Nucleosomes contain histone octamers around which DNA is wrapped [1]
Trichostatin A (TSA)-treated and enlarged cells showed similar changes in nucleosome occupancy in five out of six positions in the gene promoters. These results suggest that changes in nucleosome formation at the gene promoters could serve as stress response mechanisms elicited in response to spheroplast and TSA treatment
If nucleosome position did not change in a TSA concentration-dependent manner, at which concentration did the position change? In this study, we investigated whether genes that are known to be regulated in response to TSA treatment exhibit changes in nucleosome formation at the gene promoters in a TSA concentration-dependent manner
Summary
Nucleosomes contain histone octamers around which DNA is wrapped [1]. Neighboring nucleosomes are separated by unwrapped linker DNA. A nucleosome’s position with respect to the gene promoter plays an important role in yeast gene expression [2,3,4,5]. TSA influences nucleosome structure via histone acetylation. TSA influences nucleosome positions in the filamentous ascomycete Aspergillus fumigatus [8]. The acetylation and deacetylation of histones play an important role in the regulation of transcription [9]. Our previous study showed that TSA influences gene expression and nucleosome position in the archiascomycete Saitoella complicata [10]. Most of nucleosome positions did not change after TSA treatment [10]. The anamorphic and saprobic budding yeast S. complicata, which is classified under Taphrinomycotina, represents the earliest ascomycetous lineage [11,12]. The fission yeast Schizosaccharomyces is classified under Taphrinomycotina [12]
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