CHANGES IN MORPHOLOGICAL PARAMETERS OF BLOOD IN RABBITS DURING GASTRIC SURGERY

Abstract

The study of the reaction of the inflammatory process and its nature, depending on the use of drug treatment and without it, was carried out using a study of general blood analysis in rabbits after gastric surgery. In this study, we determined changes in the leukocyte formula of rabbits after surgery. The research material was a general blood test, namely the leukocyte formula of two groups of rabbits, each of which consisted of 5 heads. A general blood analysis study was performed on a Mindrai BC-5000 device, but white blood cells were counted manually, using a Goryaev chamber and Türk's solution, as well as a Sigeta Biogenic LED microscope (China). The leukocyte formula was calculated manually according to the Schilling formula and preliminary staining of blood smears according to the Romanovsky-Giemz method. In the study, the course of the inflammatory process in the postoperative period of rabbits was determined, which was characterized by general leukocytosis on the 3.7th day, due to an increase in neutrophils, and a left-sided followed by a right-sided shift in the leukocyte formula. According to the results of the study, it was found that the course of the inflammatory process was less pronounced in the group that received treatment.Leukocytosis with changes in the leukocyte formula of the first group of rabbits stabilized on the 10th day, in contrast to the second group that did not receive treatment. The course of the leukocytosis process was due to lymphocytopenia and an increase in the level of neutrophils, which alternated due to a previous increase in rod-shaped neutrophils, followed by a period of their decrease and an increase in the number of segmented neutrophils, which indicated the physiological course of the inflammatory process and the absence of the development of a septic process in the bodies of experimental animals.