Changes in macroelement content of the media and in sweet cherry Inmil GM 9 shoots during in vitro culture

Abstract

SummaryThe paper presents an interrelationship between macroelement content in the culture medium, their uptake from the medium, the content in the explants, as well as multiplication and the in vitro growth of the new rootstock for sweet cherry Inmil GM 9. Cultures were grown on Murashige and Skoog (MS) medium, on MS medium with double-strength, half-strength and quarter-strength macro salts with 4.4.µM BA, 0.5.µM NAA, and 0.3.µM GA3. All chemical analyses and multiplication parameters were monitored on day 0, day 20 and day 40 of culturing. As fresh and dry weight of the explants increased during subculturing, the fresh and dry weight of the media decreased. The pH of the media declined during subculturing, followed by an increase, particularly on MS 1/2 and MS 1/4 media. The genotype Inmil GM 9 showed the best growth and developmenton MS medium, where the N and P uptake was highest. The highest uptake of ions by shoots was recorded on the media with reduced macroelement content, with the exception of phosphorus. The shoots had the highest content of macroelements on MS, except for N which was highest in the explants grown on MS 2x. Leaves are mainsites of macroelement accumulation.