Abstract
Sweet cherry rootstock Gisela 5 was micropropagated on Murashige and Skoog (MS) medium, on MS medium containing double-strength macro salts (MS 2×), 1/2 strength (MS 1/2) and 1/4 strength (MS 1/4) with 4.4 μM BA, 0.5 μM NAA, and 0.3 μM GA3. All the analyses and parameters were monitored on day 0, day 20 and day 40 after subculturing. As fresh and dry weight of the explants increased during subculturing, the fresh and dry weight of the media decreased. The pH of the media declined during subculturing following by slow increase on media MS 1/2 and MS 1/4. Gisela 5 showed the best growth and development on MS 2× and MS media with the highest N and P uptake. Growth and multiplication depend on the uptake of these elements from the medium.
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