Changes in in vitro translated polypeptides of Pharbitis nil Chois., strain Violet cotyledons during the first flower-inductive and non-inductive photoperiods

Abstract

Two-dimensional gel electrophoresis was used to compare the changes between the in vitro translated polypeptides of cotyledons of Pharbitis nil Chois., strain Violet under different conditions. Seedlings were grown under short day (flower-inductive), or night break (non-inductive), or long day (non-inductive) conditions. Cotyledons were harvested every 2 h for 24 h commencing from the beginning of the dark period. Total RNA samples extracted from cotyledons were translated in vitro with a reticulocyte lysate containing [ 35S]methionine. The translation products were analyzed by two-dimensional polyacrylamide gel electrophoresis. Several differences were observed between the short day or night break treatment and long day treatment, but only two differences were detected between short-day(SD)- and night break(NB)-treated cotyledons. Their significance in the control of flowering is discussed.