In vitro translated polypeptides of different organs of Pharbitis nil Chois., strain violet under flower-inductive and non-inductive conditions

Abstract

Using two-dimensional gel electrophoresis, in vitro translated polypeptides of different organs of Pharbitis nil Chois., strain Violet were examined. Seedlings were treated with either short day (flower-inductive conditions) or night break (non-inductive conditions). Total RNA was extracted from apical buds (with leaf primordia), 1st leaves, cotyledons, cotyledonous petioles, hypocotyls, and roots. Total mRNA was translated in vitro with a rabbit reticulocyte lysate, and the translation products were analyzed by two-dimensional polyacrylamide gel electrophoresis. Differences in the mRNA of buds were detected between induced and non-induced seedlings. Several qualitative and quantitative differences were also observed among different organs that were independent of induction.