Abstract
We report on a change in the number of adsorbed glucose oxidase molecules depending on both salt concentration and illumination of a silicon substrate during glucose oxidase adsorption on its surface. Glucose oxidase molecules were adsorbed from its aqueous solution onto a silicon substrate either with a polyethyleneimine layer or without it. Based on obtained atomic force microscopy (AFM) data, it was found that addition of salt led to a more prominent effect of photo-assisted adsorption. In addition, illumination led to a monotonic change in adsorbed enzyme coverage (either decrease or increase depending on silicon substrate conductivity type), while variation of salt concentration resulted in the nonmonotonic change. The obtained results can be used for fabrication of biosensors with easily controllable parameters such as sensitivity, lower limit of analytic detection, etc.
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