Abstract
The closed circular intracellular DNA isolated from Pseudomonas BAL 31 20 min after infection with PM2 phage contains 5% catenated forms. The existence of catenated dimers is demonstrated by isolation of this species as a middle band in a propidium iodide—CsCl gradient, by electron microscopy and by sedimentation studies. The sedimentation patterns of the doubly closed dimer and the singly closed dimer in both neutral and alkaline media have been investigated in detail. The sedimentation pattern of the doubly nicked species has been examined in a neutral medium. Observed sedimentation coefficients are in sufficiently good agreement with those calculated from an existing empirical relationship to extend the useful range of applicability of this relationship to higher superhelix densities and lower molecular weights.
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